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KMID : 0895820160260020029
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Journal of Oriental Rehabilitation Medicine
2016 Volume.26 No. 2 p.29 ~ p.50
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The Effects of Gyejibokryeong-hwan (桂枝茯?丸) for Wound Healing after Skin Suture
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Jung Hoon
Lee Eun-Jung
Oh Min-Seok
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Abstract
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Objectives : There is few Korean medicinal studies about post-operation wound healing despite much effort for minimizing wound or post-op scar. The aim of this study is to evaluate the wound healing effect of Gyejibokryeong-hwan (Guizhifuling-wan, GBH) after skin suture.
Methods :＜ In vitro ＞ We observed anti-oxidative and anti-inflammatory effect by using lipopolysaccharide (LPS)-treated RAW 264.7 cells. For anti-oxidation, we mesured the total amount of polyphenol, flavonoid, DPPH scavenging ability, ABTS scavenging ability and the value of ROS production, and for anti-inflammation, we mesured the amount of NO and pro-inflammatory cytokines (TNF-α, IL-1β, IL-6). ＜ In vivo ＞ Thirty SD rats were divided into five equal groups (n=6, one normal, two controls and two experimentals). All groups except normal group were made a scar (around 1x4 cm2) in the back by the depth of the fascia and then sutured by a thread and needle. Normal group rats received no treatment at all. Control group rats were fed distilled water, and positive control group rats were percutaneously applied terramycin once in 2 days. GBH 200 group rats were orally medicated GBH 200 mg/kg, and GBH 400 group rats were orally medicated GBH 400 mg/kg per day for two weeks. We analyzed the blood samples (WBC, neutrophil, lymphocyte, monocyte, eosinophil), and the serums (TIMP-1, MMP-2, MMP-2. PGE2, TGF-β, VEGF), and examined the wounded skin tissue histopathologically.
Results :＜ in vitro ＞ 1. DPPH and ABTS scavenging activity was increased concentration-dependantly, and ROS production was significantly increased in GBH treated cells (100 μg/ml). Therefore in this study, Gyejibokryeong-hwan appears to have the anti- oxidative. 2. NO production was significantly reduced in GBH treated cells (100 μg/ml), and IL-1β production was significantly reduced in GBH treated cells (1 μg/ml). But, TNF- α and IL-6 did not show uneffective action. Therefore in this study, Gyejibokryeong-hwan did not show any significant effect on anti-inflammatory process. ＜ in vivo ＞ 1. Monocyte and neutrophil was significantly increased in GBH (200, 400) groups. WBC, lymphocyte and eosinophil did not show significant change. 2. TIMP-1, MMP-2, VEGF were significantly increased in GBH 400 group, PGE2 was significantly reduced in GBH 400 group. TGF-β was significantly increased in GBH (200, 400) groups, and MMP-9 was increased concentration-dependantly in GBH groups, but there was no significance. 3. In histopathological examinations, collagen was significantly increased and keratin was significantly decreased in GBH (200, 400) groups.
Conclusions : According to in vitro experiment, GBH appears to have the anti-oxidative effect and in vivo experiment, GBH stimulate the wound healing process hematologically and histopathologically. In conclusion, the results suggest that GBH promotes wound healing after skin suture.
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KEYWORD
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Gyejibokryeong-hwan, Guizhifuling-wan, wound healing, suture
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